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Activities of the F₁-ATPase purified from Lentinus edodes were stimulated by Fe^(2+), Fe^(2+), Cd^(2+), Mg^(2+), K^+ and Co^(2+) but were inhibited by Zn^(2+), Ca^(2+), Cu^(2+) and Ni^(2+) ion. The enzyme activities were increased 130, 65, 65, 68, 105% and 23% by the 5 mM Fe^(3+), 10 mM Fe^(2+), 1 mM Cd^(2+), 5 mM Mg^(2+), 5 mM K^+ and 5 mM Co^(2+) ion addition, respectively, as compared with those not added. The enzyme activities were decreased 18, 19, 27 and 30% by 10 mM Zn^(2+), 10 mM Ca^(2+), 0.5 mM C^(2+) and 10 mM Ni^(2+) ion, respectively. .onion effects of 10 mM CO₃^(2+), 20 mM CN^-, 20 mM CH₃COO^- and 20 mM NO₃^- ion were inhibited to the enzyme activities of 98, 95, 70 and 50%, respectively. As increasing of SO₄^(2+) ion concentration, the enzyme activity was stimulated and 20 mM SO₄^(2+) ion was shown increased of 21%.
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